EGFRvIII Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1594161
• Category: Antibodies
• Description: EGFRvIII Rabbit Polyclonal Antibody (BF647)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EGFRvIII (11-120/1081aa)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=3μg/Test, IF=1:100-500, ICC/IF=1:100
• Conjugation: BF647
• Target: EGFR
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GFR vIII; EGFR; Epidermal growth factor receptor v
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: SH-SY5Y, Primary Antibody (green line): Rabbit Anti-EGFRvIII antibody (orb1594161), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-EGFRvIII Polyclonal Antibody, conjugated (orb1594161) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.