EGFR (Phospho-Y1092) antibody

• Catalog Number: orb393269
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR (Phospho-Y1092)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1092 of human EGFR protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EGFR
• Entrez: 1956, 13649
• UniProt ID: Q01279, P00533
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR (Phospho-Y1092) expression in HEK293T (A) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)

Immunohistochemical analysis of EGFR (Phospho-Y1092) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.