EGFR Antibody

• Catalog Number: orb1261266
• Category: Antibodies
• Description: EGFR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 170kDa
• Target: EGFR
• UniProt ID: P00533
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Epidermal growth factor receptor, Proto-oncogene c
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EGFR antibody (orb1261266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using EGFR antibody (orb1261266) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using EGFR antibody (orb1261266) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using EGFR antibody (orb1261266) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord endothelial cells using EGFR antibody (orb1261266) at dilution of 1:150 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug EGFR antibody (orb1261266). Western blot was performed from the immunoprecipitate using EGFR antibody (orb1261266) at a dilution of 1:1000.