EGF Receptor / EGFRvIII Antibody

• Catalog Number: orb606694
• Category: Antibodies
• Description: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GFR/2600R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Amino acids LEEKKGNYVVTDHC were used as the immunogen for the recombinant EGFRvIII antibody. This is the predicted sequence of the exon 2-7 delete fusion region.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant EGFRvIII antibody is available for research use only.
• UniProt ID: P00533
• Storage: Store the recombinant EGFRvIII antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant EGFRvIII antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.

IHC testing of FFPE human placenta with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.

SDS-PAGE analysis of purified, BSA-free recombinant EGFRvIII antibody (clone GFR/2600R) as confirmation of integrity and purity.