EEC1 Antibody

• Catalog Number: orb1672149
• Category: Antibodies
• Description: EEC1 Antibody
• Clonality: Recombinant
• Clone Number: BBM.1
• Tested applications: Depletion, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Molt 4, a human T cell line.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: EEC1
• UniProt ID: P61769
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P, BB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of BBM.1 (orb1672149) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672149- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1. HeLa cells were stained with unimmunized r antibody (black line) or the rmeric version of BBM.1 (orb1672149 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Western Blot using anti-Beta-2 microglobulin antibody BBM.1. HeLa cell lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of BBM.1 (orb1672149) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. orb1672149 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.