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Catalog Number | orb1929808 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19285 |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Equine, Porcine, Rabbit, Rat |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 49644 Da |
Target | This EDNRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human EDNRB. |
UniProt ID | P24530 |
NCBI | NP_001116131.1, NP_000106.1, NP_003982.1, NP_001188326.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Endothelin B receptor, ET-B, ET-BR, Endothelin rec Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
EDNRB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of EDNRB Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EDNRB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of EDNRB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of EDNRB Antibody (C-term) in mouse cerebellum tissue lysates (35 ug/lane). EDNRB (arrow) was detected using the purified Pab.