EasyStep Mouse T4(Thyroxine) ELISA Kit

• Catalog Number: orb1950005
• Category: Assays and Kits
• Description: EasyStep Mouse T4(Thyroxine) ELISA Kit
• Reactivity: Mouse
• Range: 9.38-600 ng/mL
• Sensitivity: 7 ng/mL
• Alternative names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothy
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 antigen, and the Mouse T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma
• Expiration Date: Please enquire.