EasyStep Human FE(Ferritin) ELISA Kit

• Catalog Number: orb1950184
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE, and the Human FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human FE. After TMB substrate solution is added, only those wells that contain Human FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Sensitivity: 3.2 pg/mL
• Alternative names: Ferritin
• Note: For research use only
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE, and the Human FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human FE. After TMB substrate solution is added, only those wells that contain Human FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum
• Expiration Date: Please enquire.