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Catalog Number | orb1950009 |
---|---|
Category | Assays and Kits |
Description | EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
Reactivity | Human |
Range | 78.13-5000 ng/mL |
Sensitivity | 7.7 ng/mL |
Alternative names | 25 Hydroxy Vitamin D; Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, plasma |
Expiration Date | Please enquire. |
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