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Catalog Number | orb1495100 |
---|---|
Category | Proteins |
Description | E8L Recombinant Protein |
Species/Host | E. coli |
Tag | His-tag;Sumo-Tag |
MW | ~39kDa |
UniProt ID | Q5IXS0 |
Solubility (25°C) | 2M Urea, PH7.4 |
Protein Sequence | HMPQQLSPINIETKKAISDARLKTLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSTIHIYWGKEDDYGSNHLIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGIIIIAIFLQVSDHKNVYFQKIVNQLDSIRSANMSAPFDSVFYLDNLLPSTLDYFTYLGTTINHSADAAWIIFPTPINIHSDQLSKFRTLLSSSNHEGKPHYITENYRNPYKLNDDTQVYYSGEIIRAATTSPVRENYFMKWLSDLREVCFSYYQKYIEGNKLE |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Buffer/Preservatives | 2M Urea, pH 7.4 |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, SDS-PAGE, WB | |
> 90% as determined by SDS-PAGE | |
34.94 kDa | |
Mammalian Cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 32.7 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus E8L-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, SDS-PAGE, WB | |
Unconjugated | |
> 90% as determined by SDS-PAGE | |
33.92 kDa |
ELISA, SDS-PAGE, WB | |
Unconjugated | |
> 90% as determined by SDS-PAGE | |
34.94 kDa |
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