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Catalog Number | orb1463821 |
---|---|
Category | Proteins |
Description | Recombinant E8L protein |
Tested applications | ELISA, WB |
Reactivity | Virus |
Tag | N-terminal His Tag |
Purity | Greater than 90% by SDS-PAGE gel analyses |
MW | 33.7 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
UniProt ID | Q8V4Y0 |
Source | E.Coli |
Expression System | 1-275 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
Alternative names | E8L Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
ELISA, SDS-PAGE, WB | |
> 90% as determined by SDS-PAGE | |
34.94 kDa | |
Mammalian Cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 32.7 kDa after removal of the signal peptide. The apparent molecular mass of Monkeypox virus E8L-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, SDS-PAGE, WB | |
Unconjugated | |
> 90% as determined by SDS-PAGE | |
33.92 kDa |
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