E2F4 Antibody

• Catalog Number: orb1527158
• Category: Antibodies
• Description: E2F4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 363 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E2F4
• UniProt ID: Q16254
• NCBI: NP_001941.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: transcription factor E2F4; E2F transcription facto
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human E2F4 by western blot. Samples: Whole cell lysate from HeLa (5, 15, 50 µg) and HEK293T (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody orb1527158 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody orb1527158 used for IP at 6 µg per reaction.

Detection of human E2F4 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 (Cat. No. orb1527158) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB