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Catalog Number | orb576521 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to E2F3 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Rabbit, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3 |
Concentration | 0.5 mg/ml |
Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Conjugation | Unconjugated |
MW | 49 kDa |
Target | E2F3 |
UniProt ID | Q68DT0 |
Protein Sequence | Synthetic peptide located within the following region: LLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEK |
NCBI | CAH18140 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Alternative names | E2F-3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. The peptide sequence is also present in isoforms of 42 kDa and 37 kDa.
Host: Mouse, Target Name: E2F3, Sample Tissue: Mouse Small Intestine, Antibody Dilution: 1 ug/ml.
Host: Rabbit, Target Name: E2F3, Sample Tissue: Human Lung Tumor, Antibody Dilution: 1 ug/ml.
Host: Rabbit, Target Name: E2F3, Sample Tissue: Human Ovary Tumor, Antibody Dilution: 1.0 ug/ml.
Host: Rabbit, Target Name: E2F3, Sample Tissue: Human OVCAR-3 Whole Cell, Antibody Dilution: 0.5 ug/ml.
Host: Rabbit, Target Name: E2F3, Sample Tissue: Human OVCAR-3 Whole Cell, Antibody Dilution: 1 ug/ml.
WB Suggested Anti-E2F3 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human Thymus.
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Canine, Equine, Human, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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