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Catalog Number | orb534005 |
---|---|
Category | Antibodies |
Description | Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | ECD1-3R |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1) |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant E-Cadherin antibody is available for research use only. |
UniProt ID | P12830 |
Storage | Store the recombinant E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).
Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone ECD1-3R). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Formalin-fixed, paraffin-embedded human cervix stained with recombinant E-Cadherin antibody (clone ECD1-3R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R); Red=isotype control, Blue=recombinant E-Cadherin antibody.
FACS, IF, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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