DYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2)

• Catalog Number: orb1294275
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DYKDDDDK tag
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide (DYKDDDDK)
• Dilution range: Western Blot 1:3000-1:5000 Immunofluorescence 1:500-1:1000
• Conjugation: Unconjugated
• Target: DYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti DYKDDDDK-tag antibody at 1/3000 dilution, Lysates/proteins at 35 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294275 overnight at 4°C.