DYKDDDDK (FLAG) Positive Control Lysate

• Catalog Number: orb348661
• Category: Tools
• Description: DYKDDDDK (FLAG) Positive Control Lysate
• Species/Host: Rabbit
• Tested applications: SDS-PAGE, WB
• Concentration: 1.0 mg/ml
• Dilution range: WB: Yes - User Optimized
• Form/Appearance: Liquid
• Purity: Lysates are supplied in 1X loading buffer and is ready-to-use. Supplied in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• Storage: Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
• Buffer/Preservatives: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Alternative names: DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK
• Note: For research use only
• Application notes: ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μl is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
• Expiration Date: 12 months from date of receipt.

Western Blot of DYKDDDDK (FLAG®) Positive Control Lysate. Lane 1: DYKDDDDK (FLAG®) Positive Control Lysate. Load: 10 µg per lane. Primary antibody: Flag Antibody at 1:1000 overnight at 4°C. Secondary antibody: Mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 60 kDa for DYKDDDDK tagged recombinant protein.

Western Blot of DYKDDDDK (FLAG®) Positive Control Lysate. Lane 1: Opal Pre-stained Protein Standard 10-245 kDa. Lane 2: FLAG positive control lysate (p/n orb348661) 10 ug. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1 hr at RT. Detection Antibody: Anti-FLAG DyLight 488 at 1:10000 for 1 hr at RT. Expect a band ~64 kDa.