DVL1 Antibody (Center)

• Catalog Number: orb1787884
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 442-470 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:2000
• Conjugation: Unconjugated
• MW: 75187 Da
• Target: This DVL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the Central region of human DVL1.
• UniProt ID: O14640
• NCBI: NP_004412.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Segment polarity protein dishevelled homolog DVL-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

All lanes: Anti-DVL1 Antibody (Center) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: MDA-MB453 whole cell lysate. Lane 5: human kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.