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Catalog Number | orb308875 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to DUT |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Human, Porcine |
Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 26.6; 17.7; 15.4 |
Target | dUTPase / DUT |
Entrez | 1854 |
Protein Sequence | QLRFARLSEHATAPT |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | DUT; deoxyuridine triphosphatase; dUTPase; dUTP nu Read more... |
Note | For research use only |
Application notes | WB: Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5µg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 (Lane 1) and HeLa (Lane 2) lysates using DUT antibody
Immunohistochemical staining of Human Testis using DUT antibody
Immunohistochemical staining of Human Tonsil using DUT antibody
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Porcine | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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