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Catalog Number | orb1258016 |
---|---|
Category | Antibodies |
Description | DUT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:100 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 24kDa |
Target | DUT |
UniProt ID | P33316 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | DUT, FLJ20622, dUTPase Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:100 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DUT antibody (orb1258016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (orb1258016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Porcine | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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