dsDNA Antibody / Double Stranded DNA

• Catalog Number: orb749958
• Category: Antibodies
• Description: Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 121-3
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG3, kappa
• Immunogen: Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This dsDNA antibody is available for research use only.
• UniProt ID: Not Known
• Storage: Store the dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone 121-3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with dsDNA antibody (clone 121-3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of fixed and permeabilized human Jurkat cells with dsDNA antibody (clone 121-3); Red=isotype control, Blue=dsDNA antibody.