dsDNA Antibody / Double Stranded DNA

• Catalog Number: orb1822663
• Category: Antibodies
• Description: This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rDSD/8266
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Burkitt's cell nuclei were used as the immunogen for the Double Stranded DNA antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Double Stranded DNA antibody is available for research use only.
• Storage: Aliquot the Double Stranded DNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human ovarian cancer tissue with Double Stranded DNA antibody (clone rDSD/8266). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Double Stranded DNA antibody (clone rDSD/8266) as confirmation of integrity and purity.