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Catalog Number | orb1822661 |
---|---|
Category | Antibodies |
Description | This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rDSD/8266 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Burkitt's cell nuclei were used as the immunogen for the Double Stranded DNA antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Double Stranded DNA antibody is available for research use only. |
Storage | Aliquot the Double Stranded DNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human ovarian cancer tissue with Double Stranded DNA antibody (clone rDSD/8266). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Double Stranded DNA antibody (clone rDSD/8266) as confirmation of integrity and purity.
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
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