Donkey IgG (H&L) Antibody

• Catalog Number: orb347992
• Category: Antibodies
• Description: Donkey IgG (H&L) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Donkey
• Isotype: IgG
• Immunogen: Donkey IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG and Donkey Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Donkey IgG Antibody, goat anti Donkey Ig
• Note: For research use only
• Application notes: Anti-Donkey IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Goat Anti-Donkey IgG Antibody tested against purified Donkey IgG. Each well was coated in duplicate with 1.0 µg of Donkey IgG (p/n orb2652734). The working dilution is 1:38200. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).