Donkey anti-Rat IgG (H&L) antibody (HRP)

• Catalog Number: orb700045
• Category: Antibodies
• Description: Donkey polyclonal antibody against Donkey anti-Rat IgG (H&L) conjugated to HRP
• Species/Host: Donkey
• Clonality: Polyclonal
• Dilution range: The optimal working dilution should be determined by the investigator
• Form/Appearance: Lyophilized
• Purity: Immunogen affinity purified IgG.
• Conjugation: HRP
• Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only
• Application notes: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis, except rat Ig1.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
• Expiration Date: 12 months from date of receipt.