Donkey Anti-Rabbit IgG H&L, BF647 conjugated

• Catalog Number: orb1817041
• Category: Antibodies
• Description: Donkey Anti-Rabbit IgG H&L, BF647 conjugated
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: FC, IF
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Native rabbit IgG
• Concentration: 2.0 mg/ml
• Dilution range: IF=1:100-1000, Flow-Cyt=1:100-1000
• Conjugation: BF647
• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Alternative names: Donkey Anti-Rabbit IgG H&L (BF647); Immunoglobulin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.