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Catalog Number | orb1258696 |
---|---|
Category | Antibodies |
Description | DNMT1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein of Human DNMT1. |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 200kDa |
Target | DNMT1 |
UniProt ID | P26358 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using DNMT1 antibody (orb1258696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ChIP, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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