Dnm1 Antibody

• Catalog Number: orb1273529
• Category: Antibodies
• Description: Dnm1 Antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Dynamin (Ser778) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser778 of human, mouse and rat dynamin.
• Concentration: batch dependent
• Dilution range: Dynamin antibody for phospho-Ser778 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser778) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 95
• Target: Dnm1
• UniProt ID: P21575
• NCBI: P21575
• Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
• Note: For research use only
• Application notes: Dynamin antibody for phospho-Ser778 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser778) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for WB.
• Expiration Date: 12 months from date of receipt.

Western blot of a forskolin stimulated rat hippocampal lysate showing phospho-specific immunolabeling of the ~95k Dynamin protein phosphorylated at Ser778.