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Catalog Number | orb1239023 |
---|---|
Category | Antibodies |
Description | DNASE2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II.The immunogen is located within the last 50 amino acids of DNase II. |
Concentration | 1 mg/ml |
Dilution range | DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Aprox: 40 kDa |
Target | DNASE2 |
UniProt ID | O00115 |
NCBI | AF047016 |
Storage | DNase II antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | DNase II Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonu Read more... |
Note | For research use only |
Application notes | DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.
Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 µg/mL.
Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml. Green: DNase II antibody (orb1239023) Red: Phylloidin staining Blue: DAPI staining.
Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml.
WB | |
Bovine, Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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