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| Catalog Number | orb1806547 |
|---|---|
| Category | Assays and Kits |
| Description | DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit |
| Reactivity | All |
| Range | 156.25-10000 pg/mL |
| Target | DHVD3 |
| Sensitivity | 49.5 pg/mL |
| Alternative names | DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH) Read more... |
| Note | For research use only |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,25-Dihydroxyvitamin D3(DHVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3(DHVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,25-Dihydroxyvitamin D3(DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Sample Types | Serum, Plasma and Other biological fluids |
| Expiration Date | Please enquire. |
