DHRS9 Antibody

• Catalog Number: orb1257093
• Category: Antibodies
• Description: DHRS9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 35kDa
• Target: DHRS9
• UniProt ID: Q9BPW9
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, R
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DHRS9 antibody (orb1257093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 390s.

Western blot analysis of extracts of various cell lines, using DHRS9 antibody (orb1257093) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (orb1257093) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (orb1257093) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using DHRS9 antibody (orb1257093). Blue: DAPI for nuclear staining.