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Catalog Number | orb1239262 |
---|---|
Category | Antibodies |
Description | Dffb Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Human, Rat |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.The immunogen is located within the last 50 amino acids of CAD. |
Concentration | 1 mg/ml |
Dilution range | CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 40 kDa |
Target | Dffb |
UniProt ID | O54788 |
NCBI | O54788 |
Storage | CAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | CAD Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | CAD Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730 Read more... |
Note | For research use only |
Application notes | CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution.
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 µg/mL.
Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 µg/mL.
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml.
Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml. Green: CAD antibody (orb1239262) Red: Phylloidin staining Blue: DAPI staining.
ELISA, IF, IHC-P, WB | |
Bovine, Human | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Bovine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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