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Catalog Number | orb1239028 |
---|---|
Category | Antibodies |
Description | Dffb Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD.The immunogen is located within amino acids 130 - 180 of DFF40. |
Concentration | 1 mg/mL |
Dilution range | DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 38 kDa Observed: 40 kDa |
Target | Dffb |
UniProt ID | O54788 |
NCBI | NP_031885 |
Storage | DFF40 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | DFF40 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | DFF40 Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 57 Read more... |
Note | For research use only |
Application notes | DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.
Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/mL.
Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/ml.
Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL. Green: DFF40 Antibody (orb1239028) Blue: DAPI staining.
ELISA, IF, IHC-P, WB | |
Bovine, Human | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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