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Catalog Number | orb1239883 |
---|---|
Category | Antibodies |
Description | DFFA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45.The immunogen is located within the last 50 amino acids of DFF45. |
Concentration | 1 mg/ml |
Dilution range | DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 45 kDa |
Target | DFFA |
UniProt ID | O00273 |
NCBI | NP_004392 |
Storage | DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | DFF45 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H Read more... |
Note | For research use only |
Application notes | DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.
Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 µg/mL.
Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 µg/mL. Green: DFF45 Antibody (orb1239883) Blue: DAPI staining.
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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