Dextranase (Penicillium Species) Antibody Biotin Conjugated

• Catalog Number: orb344751
• Category: Antibodies
• Description: Dextranase antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Algae
• Isotype: IgG
• Immunogen: Dextranase [Penicillium species] DEX
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P48845
• NCBI: AAB47720.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Dextranase Antibody biotin Conjugation
• Note: For research use only
• Application notes: Anti-Dextranase Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Dextranase Antibody Biotin Conjugated tested against purified Dextranase protein. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).

Western Blot of Anti-Dextranase Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase Biotin Conjugate at 1:1000 overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~66kDa.