Desmoglein 3 antibody

• Catalog Number: orb2276284
• Category: Antibodies
• Description: Desmoglein 3 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-543M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)
• Dilution range: 1:75-1:150
• Conjugation: Unconjugated
• UniProt ID: P32926
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: 130kDa pemphigus vulgaris antigen (PVA); Balding (
• Note: For research use only
• Application notes: Positive Control: Tonsil: A moderate to strong desmoglein 3 immunostaining should be seen in a fraction of squamous epithelial cells. Negative Control: Tonsil: Desmoglein 3 immunostaining should be absent in all non-squamous epithelial cell types. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Esophagus squamous epithelium In the esophagus a strong membranous Dsg3 immunostaining predominantly occurs in the lower 2/3 of the squamous epithelium.

Squamous cell carcinoma of the penis with intense membranous Dsg3 immunostaining of tumor cells.

Tonsil In the tonsil a moderate to strong Dsg3 immunostaining occurs in squamous epithelial cells of crypts. All other cell types are Dsg3 negative.