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Catalog Number | orb1824867 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This mAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | DSG3/8613R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the Desmoglein 3 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Desmoglein 3 antibody is available for research use only. |
UniProt ID | P32926 |
Storage | Aliquot the Desmoglein 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human skin tissue with Desmoglein 3 antibody (clone DSG3/8613R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Desmoglein 3 antibody (clone DSG3/8613R) as confirmation of integrity and purity.
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