Desmoglein 2 antibody

• Catalog Number: orb579404
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Desmoglein 2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DSG2
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 114 kDa
• Target: DSG2
• UniProt ID: Q14126
• Protein Sequence: Synthetic peptide located within the following region: KIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREE
• NCBI: NP_001934
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: HDGC, CDHF5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Host: Rabbit, Target: DSG2, Positive control (+): 293T Cell Lysate (2T), Negative control (-): Human Brain (BR), Antibody concentration: 1 ug/ml.

Immunohistochemistry with Human Colon lysate tissue at an antibody concentration of 5.0 ug/ml using anti-DSG2 antibody (orb579404).

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-DSG2 Antibody Titration: 1 ug/ml, Positive Control: Hela cell lysate.