Desmin antibody

• Catalog Number: orb1151455
• Category: Antibodies
• Description: Mouse monoclonal antibody to Desmin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DES/3255
• Tested applications: IHC-P
• Reactivity: Bovine, Canine, Equine, Feline, Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human desmin protein was used as the immunogen for the Desmin antibody.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Desmin antibody is available for research use only.
• UniProt ID: P17661
• Storage: Aliquot the Desmin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Desmin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cardiac muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skeletal muscle with Desmin antibody (clone DES/3255) at 2 ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog smooth muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat smooth muscle tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse smooth muscle tissue withe Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cow small intestine tissue with Desmin antibody (clone DES/3255) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Desmin antibody (clone DES/3255) as confirmation of integrity and purity.