DENND1B Antibody

• Catalog Number: orb1270847
• Category: Antibodies
• Description: DENND1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: DENND1B
• UniProt ID: Q6P3S1
• NCBI: Q6P3S1
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: DENN domain-containing protein 1B, Connecdenn 2, P
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane).

DENND1B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.