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Catalog Number | orb260202 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Dengue NS1 |
Clonality | Monoclonal |
Reactivity | Virus |
Form/Appearance | 2 vials of sterile Filtered clear colorless solution. |
Purity | 95% pure (12% SDS-PAGE and Coomassie staining). |
Conjugation | Unconjugated |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | *Dengue NS1 Antibody solution for gold conjugation (3.6mg/ml) in PBS and Sodium nitrate. *Dengue NS1 Antibody solution for membrane coating (2.2mg/ml) in PBS and Sodium nitrate. |
Note | For research use only |
Application notes | Applications: Lateral flow immunoassay. Assay Conditions: 1. 1-2 days fresh prepared gold particle with maximal homogeneity in gold particle size, λ 525-529, OD 1.2-1.4. 2. Conjugation at pH 8.5, conjugation time should be 6-8 hours. 3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0- 8.2, add casein to the final 0.3-0.4%. 4. Treat sample pad with 50mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH8.0 |
Expiration Date | 12 months from date of receipt. |
Human | |
1.57-100 ng/mL | |
0.65 ng/mL |
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