DELTA-1 antibody

• Catalog Number: orb345496
• Category: Antibodies
• Description: DELTA-1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DELTA-1. Swiss Prot accession Q61483.
• Concentration: 1.21 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:12,000, IHC: 2ug/ml, WB: 1:100 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-DELTA-1 Antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. The antibody detects DELTA-1 from mouse. Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.
• Conjugation: Unconjugated
• UniProt ID: Q61483
• NCBI: NP_031891.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Delta1 antibody, Delta-1, Delta 1, Del
• Note: For research use only
• Application notes: Anti-DELTA-1 Antibody has been tested for use in ELISA against the immunizing peptide, western blot, and in IHC. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Rabbit anti-Mouse DELTA-1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Immunohistochemistry of Rabbit Anti-Delta1 antibody. Tissue: Mouse embryonic kidney. Fixation: formalin fixed paraffin embedded 10% conc. Antigen retrieval: pH 9.5. Primary antibody: 2 ug/ml for 1.5 hour @ room Temp. Secondary antibody: Peroxidase secondary reagent for 45 min at RT. Localization: as shown. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry of Rabbit anti-mouse DELTA1 Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle). Primary antibody: 2 ug/ml 1.5 hour @ room temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45" RT.