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Catalog Number | orb1272591 |
---|---|
Category | Antibodies |
Description | DEFB4A Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2 (human BD-2). |
Concentration | batch dependent |
Dilution range | ELISA:To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBD-2. Western Blot:To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | DEFB4A |
UniProt ID | O15263 |
NCBI | O15263 |
Storage | BD-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | BD-2, SAP1, DEFB2, DEFB4, HBD-2, DEFB-2, DEFB102, Read more... |
Note | For research use only |
Application notes | ELISA:To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBD-2. Western Blot:To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human BD-2 (orb1272590) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-2.
IHC, WB | |
Human | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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