Decorin antibody (Biorbyt-537R

• Catalog Number: orb2276297
• Category: Antibodies
• Description: Decorin antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-537R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P07585
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Bone proteoglycan II; CSCD; Dermatan sulphate prot
• Note: For research use only
• Application notes: Positive Control: Fallopian tube: A strong staining should be seen in the stroma while epithelial cells remain negative. Negative Control: Fallopian tube: Epithelial cells should not show any staining. Cellular Localization: Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Fallopian tube mucosa Strong decorin immunostaining in the stroma while staining is completely lacking in epithelial cells decorin immunohistochemistry.

Serous high grade carcinoma of the ovary showing decorin staining of the stroma.

Strong decorin staining of the stroma while urothelium remains unstained in the urinary bladder.