DDX39B Antibody

• Catalog Number: orb1262532
• Category: Antibodies
• Description: DDX39B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Drosophila, Gallus, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This BAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human BAT1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: DDX39B
• UniProt ID: Q13838
• NCBI: Q13838
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Spliceosome RNA helicase DDX39B, 56 kDa U2AF65-ass
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in A431, Hela, Jurkat cell line lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.