DDIT3 antibody

• Catalog Number: orb638919
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DDIT3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, IP, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDIT3/CHOP (NP_004074.2).
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Conjugation: Unconjugated
• MW: 30kDa
• UniProt ID: P35638
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: Anti-DDIT3 antibody, anti-CEBPZ antibody, anti-CHO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using DDIT3/CHOP Rabbit pAb (orb638919) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using DDIT3/CHOP Rabbit pAb (orb638919) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunoprecipitation analysis of 300 µg extracts of C2C12 tunicamycin cells using 3 µg DDIT3/CHOP antibody (orb638919). Western blot was performed from the immunoprecipitate using DDIT3/CHOP antibody (orb638919) at a dilution of 1:500.

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (orb638919) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.