DDIT3 antibody

• Catalog Number: orb135381
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DDIT3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human DDIT3/CHOP (NP_004074.2).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-100
• Conjugation: Unconjugated
• MW: 27kDa
• UniProt ID: P35638
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Alternative names: anti C/EBP homologous protein antibody, anti C/EBP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of C6 cells using DDIT3/CHOP Rabbit pAb (orb135381) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using DDIT3/CHOP Rabbit pAb (orb135381) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit pAb (orb135381) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DDIT3/CHOP Rabbit pAb (orb135381) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DDIT3/CHOP Rabbit pAb (orb135381) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from C2C12 cells, using DDIT3/CHOP Rabbit pAb (orb135381) at 1:1000 dilution. C2C12 cells were treated by tunicamycin (2 µg/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (orb135381) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (orb135381) at 1:2000 dilution. C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of various lysates using DDIT3/CHOP Rabbit pAb (orb135381) at 1:1000 dilution. C2C12 and C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.