DCTN1 Antibody

• Catalog Number: orb1258530
• Category: Antibodies
• Description: DCTN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 945-1139 of human DCTN1 (NP_001128513.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 150kDa
• Target: DCTN1
• UniProt ID: Q14203
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: DCTN1, dynactin 1 (p150, glued homolog, Drosophila
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DCTN1 antibody (orb1258530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using DCTN1 antibody (orb1258530). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug DCTN1 antibody (orb1258530). Western blot was performed from the immunoprecipitate using DCTN1 antibody (orb1258530) at a dilution of 1:1000.