DBCO-PEG4-FLAG

• Catalog Number: orb532287
• Category: Tools
• Description: DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 µM) or labeling of Azide-functionalized micelles (amount: 100 nmol). These concentrations may serve as a starting point for individual assay set-up.
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1790.85 g/mol; Detected MW: 1789.70 g/mol
• Formula: C₈₀H₁₀₇N₁₅O₃₀S
• Solubility (25°C): water, PBS (up to 30 mM tested)
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Spectroscopic Propertie: λmax 308 nm, ε 13.8.0 L mmol-1cm-1.
• Expiration Date: 12 months from date of receipt.