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Catalog Number | orb1151770 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Daxx |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PCRP-DAXX-8C2 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG |
Immunogen | Recombinant full-length human Death domain-associated protein 6 protein was used as the immunogen for the Daxx antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Daxx antibody is available for research use only. |
UniProt ID | Q9UER7 |
Storage | Aliquot the Daxx antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Daxx antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-8C2).
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8C2), and unstained cells (red).
IHC staining of FFPE human kidney tissue with Daxx antibody (clone PCRP-DAXX-8C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8C2) as confirmation of integrity and purity.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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