Daxx antibody

• Catalog Number: orb1151654
• Category: Antibodies
• Description: Mouse monoclonal antibody to Daxx
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-DAXX-8B7
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Daxx antibody is available for research use only.
• UniProt ID: Q9UER7
• Storage: Aliquot the Daxx antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Daxx antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-8B7). These results demonstrate the foremost specificity of the PCRP-DAXX-8B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunofluorescent staining of human U87 cells using Daxx antibody (green, clone PCRP-DAXX-8B7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8B7), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8B7) as confirmation of integrity and purity.